Christopher Vollmers graduated with a M.S. in Biomedical Sciences from the University of Wuerzburg (Germany). He then pursued my Ph.D. through a shared program between the University of Heidelberg (Germany) and the Salk Institute for Biological Studies in La Jolla, CA. After graduating, he joined the lab of Stephen Quake at Stanford University as a postdoctoral fellow. In the Quake lab, he worked on developing genomic tools to analyze B cells on population and single cell levels. He now continues this work with a focus on long read sequencing technologies in his own lab which he started in 2014 in the Biomolecular Engineering Department at UCSC.
Recent advances in sequencing technology have the potential to enable a new generation of tools for bulk and single cell transcriptome analysis. In particular, long-read sequencing makes it possible to, at high throughput, sequence full-length transcripts as opposed to sequencing transcript fragments using short-read based RNA-seq. I will outline the big potential of long-read sequencing technology for transcriptome analysis and annotation and talk about my lab's work on overcoming inherent limitations of this technology.